Oxidized low-density lipoproteins enhance expression and activity of CD39 and CD73 in the human aortic valve endothelium. (1st December 2016)

• Record Type: Journal Article
• Authors: Kaniewska-Bednarczuk, Ewa; Mielcarek, Michał; Chester, Adrian H.; Slominska, Ewa M.; Yacoub, Magdi H; Smolenski, Ryszard T.
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 35:Number 10/12(2016)
• Page Start: 713
• Page End: 719
• Publication Date: 2016-12-01
• Subjects: Ox-LDL -- aortic valve -- endothelium -- CD39 -- CD73; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2016.1163377
• Languages: English
• ISSNs: 1525-7770

Abstract

ABSTRACT: Extracellular nucleotides regulate thrombosis, inflammation, and immune response. Ectonucleoside triphosphate diphosphohydrolase 1 (CD39) and ecto-5'-nucleotidase (CD73) convert extracellular nucleotides in a sequential order: ATP to ADP, AMP, and then to adenosine. In this study, we aimed to test an effect of oxidized low-density lipoprotein (ox-LDL) on CD39 and CD73 in endothelial cells. Human aortic valve endothelial cells were exposed to ox-LDL for 24–48 h. Next, the activity, protein expression, and mRNA transcripts level of CD39 and CD73 were characterized by an incubation with ATP or AMP followed by high-performance liquid chromatography analysis of media as well as western blots and qPCR. CD73 activity in human valve endothelial cells was increased in presence of ox-LDL (4.04 ± 0.32 nmol/mg prot./min, mean +/− SEM) as compared with control (2.75 ± 0.21 nmol/mg prot/min). There was almost no effect of ox-LDL on CD39 activity. A similar effect was observed for mRNA and protein expression. In conclusion, we found that ox-LDL modulated CD39 and CD73 activity in the endothelium, which may contribute to relevant pathologies and featured treatments.