Determination of carnosic acid and carnosol in Rosmarinus officinalis L. by high-performance capillary electrophoresis. Issue 3 (4th May 2017)

• Record Type: Journal Article
• Authors: Lu, Ping; Ma, Jin-qi; Li, Pu; Zhou, Hai-mei; Xu, Xing-min; Guo, Wan
• Is Part Of: Instrumentation science & technology. Volume 45:Issue 3(2017)
• Page Start: 268
• Page End: 275
• Publication Date: 2017-05-04
• Subjects: Carnosic acid -- carnosol -- high-performance capillary electrophoresis -- Rosmarinus officinalis L.; Scientific apparatus and instruments -- Periodicals; 681.2
• DOI: 10.1080/10739149.2016.1227834
• Languages: English
• ISSNs: 1073-9149

Abstract

ABSTRACT: Carnosol and carnosic acid in Rosmarinus officinalis L. were separated by high-performance capillary electrophoresis using a 50 cm capillary, a 12 mmol/L 80:20 pH 9.9 borax:methanol buffer using 25 kV of applied voltage at 30°C with detection at 280 nm. Rosmarinus officinalis L. was extracted with methanol with ultrasound at 170 W and 50 kHz for 20 min. The precision, repeatability, and recovery of the method were evaluated. The calibration relationships for carnosol and carnosic acid were y = 2.331x + 5.92 from 0.01 to 0.160 mg/mL and y = 7.980x + 4.32 from 0.005 to 0.080 mg/mL, respectively. The established method allows the separation and quantification of carnosic acid and carnosol in R. officinalis from various locations in China.