Expression profiles of two thaumatin‐like protein (TmTLP) genes in responses to various micro‐organisms from Tenebrio molitor. Issue 1 (12th October 2016)

• Record Type: Journal Article
• Authors: Kim, Dong Hyun; Noh, Mi Young; Park, Ki Beom; Jo, Yong Hun
• Is Part Of: Entomological research. Volume 47:Issue 1(2017:Jan.)
• Page Start: 35
• Page End: 40
• Publication Date: 2016-10-12
• Subjects: antimicrobial peptide -- expression patterns -- Tenebrio molitor -- thaumatin‐like protein; Insects -- Korea -- Periodicals; Entomology -- Periodicals; 595.709519
• DOI: 10.1111/1748-5967.12197
• Languages: English
• ISSNs: 1738-2297

Abstract

Abstract: It is well known that anti‐fungal thaumatin‐like proteins (TLPs) play important roles in plants. Here, we investigated the expression analysis of thaumatin‐like protein genes TmTLP s in response to various pathogens in Tenebrio molitor . Developmental expression patterns of TmTLP s show that TmTLP s are highly expressed in the early pupal and adult stages. Furthermore, tissue‐specific expression patterns of TmTLP s indicate that TmTLP1 is highly expressed in the integument and gut of last instar larvae and the integument and Malpighian tubules of 5‐day old adults. In contrast, TmTLP2 is highly expressed in the gut of both last instar larvae and 5‐day old adults. We hypothesize that the expression of TmTLP genes in developmental stages may be related to molting and body remodeling stresses. In addition, the induction patterns of TmTLP genes indicate that TmTLP s were slightly induced by Escherichia coli, Staphylococcus aureus, Candida albicans and Listeria monocytogenes . Furthermore, TmTLP1 and TmTLP2 were strongly induced in response to E. coli at 9 h post‐injection and L. monocytogenes at 3 h post‐injection. Our results suggest that TmTLPs may possess antimicrobial functions in T. molitor .