Enhanced Th2 cell differentiation and function in the absence of Nox2. Issue 2 (6th July 2016) - Kwon, B.‐I.
Abstract: Background: Patients with chronic granulomatous disease (CGD), whom inherit abnormal function of NADPH oxidase 2 (Nox2), suffer from hyperinflammatory responses in lung as well as bacterial and fungal infection. There have been studies to reveal the function of Nox2 in hyperinflammatory diseases, especially in asthma, but the exact role of Nox2 in asthma is still unclear and controversial. Therefore, we attempted to clarify the exact role of Nox2 in asthma, using various experimental asthma models. Methods: Asthma phenotypes were analyzed in response to various allergen‐induced experimental asthma using Nox2‐deficient mice and recombinase gene‐activating‐1‐deficient mice. To understand the underlying mechanisms of exaggerated Th2 effector functions, we investigated the degree of T‐cell activation, levels of activation‐induced cell death (AICD), and regulatory T (Treg)‐cell differentiation in Nox2‐deficient T cells. Results: Asthma phenotypes were increased through enhanced Th2 differentiation and function in Nox2‐null mice regardless of dose and route of various allergens. Nox2‐deficient T cells also showed hyperactivation, reduced AICD, and diminished Treg‐cell differentiation through increased AKT phosphorylation (T308/S473) and enhanced mitochondrial ROS production. Conclusion: Our findings indicate that Nox2 deficiency results in exaggerated experimental asthma, which is caused by enhanced Th2 effector function in a T‐cell‐intrinsic manner.