Connexin32 deficiency is associated with liver injury, inflammation and oxidative stress in experimental non‐alcoholic steatohepatitis. (February 2017)

• Record Type: Journal Article
• Authors: Tiburcio, Taynã Cristina; Willebrords, Joost; da Silva, Tereza Cristina; Pereira, Isabel Veloso Alves; Nogueira, Marina Sayuri; Crespo Yanguas, Sara; Maes, Michaël; Silva, Elisangela dos Anjos; Dagli, Maria Lucia Zaidan; de Castro, Inar Alves; Oliveira, Cláudia Pinto; Vinken, Mathieu; Cogliati, Bruno
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 44:Number 2(2017:Feb.)
• Page Start: 197
• Page End: 206
• Publication Date: 2017-02
• Subjects: connexin32 -- inflammation -- liver damage -- non‐alcoholic steatohepatitis -- oxidative stress -- steatosis; Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.12701
• Languages: English
• ISSNs: 0305-1870

Abstract

Summary: Non‐alcoholic steatohepatitis is a highly prevalent liver pathology featured by hepatocellular fat deposition and inflammation. Connexin32, which is the major building block of hepatocellular gap junctions, has a protective role in hepatocarcinogenesis and is downregulated in chronic liver diseases. However, the role of connexin32 in non‐alcoholic steatohepatitis remains unclear. Connexin32 −/− mice and their wild‐type littermates were fed a choline‐deficient high‐fat diet. The manifestation of non‐alcoholic steatohepatitis was evaluated based on a battery of clinically relevant read‐outs, including histopathological examination, diverse indicators of inflammation and liver damage, in‐depth lipid analysis, assessment of oxidative stress, insulin and glucose tolerance, liver regeneration and lipid‐related biomarkers. Overall, more pronounced liver damage, inflammation and oxidative stress were observed in connexin32 −/− mice compared to wild‐type animals. No differences were found in insulin and glucose tolerance measurements and liver regeneration. However, two lipid‐related genes, srebf1 and fabp3, were upregulated in Cx32 −/− mice in comparison with wild‐type animals. These findings suggest that connexin32‐based signalling is not directly involved in steatosis as such, but rather in the sequelae of this process, which underlie progression of non‐alcoholic steatohepatitis.