Effects of Proanthocyanidins on Intestinal Motility Disturbance Following Intestinal Ischemia/Reperfusion. (1st November 2016)

• Record Type: Journal Article
• Authors: Luo, Lan; Li, Yu-Cheng; Dai, Xiao-Zhen; Yang, Zheng; Song, Qiang; Hu, Wen-Si; Cao, De-Qi; Zhang, Xiao
• Is Part Of: Journal of investigative surgery. Volume 29:Number 6(2016:Dec.)
• Page Start: 335
• Page End: 342
• Publication Date: 2016-11-01
• Subjects: intestinal ischemia/reperfusion -- proanthocyandins -- antioxidant -- anti-apoptosis -- intestinal mucosa -- intestinal motility; Surgery -- Research -- Periodicals; Research; Surgery; Surgical Procedures, Operative; 617.075
• DOI: 10.3109/08941939.2016.1149642
• Languages: English
• ISSNs: 0894-1939

Abstract

ABSTRACT: Purpose: To investigate the potential protective effects of Proanthocyanidins(PAs) on intestinal motility disturbance following intestinal ischemia/reperfusion (I/R). Materials and Methods: Male rats were divided into four groups: Sham, I/R, I/R+PA100 and I/R+PA200. Sham group underwent laparotomy without ligation, the others were subjected to intestinal ischemia for 1 h and reperfusion 4 h. Rats in the I/R+PA100 group received PAs (100 mg/kg/d) for 5 days prior to I/R, while rats in the I/R+PA200 group received PAs (200 mg/kg/d). After reperfusion, using an electrophysiology instrument measured ileal slow wave. Ileal specimens were obtained to determine contractility, tissue levels of Bax, Bcl-2, and Caspase-3 and evaluate histopathological changes. In addition, blood sample was obtained to determine serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels. Results: Intestinal I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, and hemorrhage. Both PAs treatment decreased mucosal pathological impairment in comparison with the I/R group ( p < .05) in light microscopic evaluations. In both PAs-treated groups, Bax and Caspase-3 expression were decreased compared to I/R group, while the Bcl-2 expression increased ( p < .05), which was similarly the case for serum SOD activity demonstrated significant enhance ( p < .05) and decline in MDA levels in comparison with I/R group (both p < .05). Moreover, PAs treatment was more efficient in attenuating serum MDA levels of intestinal I/R (both p < .05). And the contractile amplitude and frequency of slow wave in I/R+PA100 and I/R+PA200 groups were higher than I/R group (both p < .05). Conclusions: PAs improve intestinal motility disturbance following intestinal I/R by alleviating oxidative stress and apoptosis.