Molecular characterization of Ambler class A to D β-lactamases, ISAba1, and integrons reveals multidrug-resistant Acinetobacter spp. isolates in northeastern China. (1st November 2016)

• Record Type: Journal Article
• Authors: Sun, Xiaoyu; Liu, Bin; Chen, Yan; Huang, Honglan; Wang, Guoqing; Li, Fan; Ni, Zhaohui
• Is Part Of: Journal of chemotherapy. Volume 28:Number 6(2016:Dec.)
• Page Start: 469
• Page End: 475
• Publication Date: 2016-11-01
• Subjects: Acinetobacter spp. -- β-lactamases -- ISAba1 -- Integron -- Northeastern China; Chemotherapy -- Periodicals; Drug Therapy -- Periodicals; 615.58
• DOI: 10.1080/1120009X.2015.1133014
• Languages: English
• ISSNs: 1120-009X

Abstract

Abstract : The prevalence of various Ambler class A to D β-lactamases, IS Aba1, and class 1 and 2 integrons as well as the clonal relatedness in 105 Acinetobacter spp. isolates found in northeastern China was investigated. All 105 Acinetobacter spp. isolates were determined to be multidrug resistant (MDR), and the resistance rates to carbapenem agents were approximately 50%. PER, IMP, AmpC, and OXA-23 were found to be dominant β-lactamases belonging to different classes, respectively. This is the first report of the coexistence of bla PER, bla IMP, bla AmpC, and bla OXA-23-like genes in Acinetobacter spp. isolates from northeastern China. IS Aba1 was found upstream of the bla OXA-23-like gene in 87.8% (36/41) strains and upstream of the bla OXA-51-like gene in 26.5% (13/49) strains. IS Aba3 –like element was found upstream of the bla OXA-58-like gene in one bla OXA-58-like -positive strain. The presence of IntI1 was detected in 63.8% (67/105) of the isolates and the most prevalent gene cassettes were aacA4, aadA1, and catB8. The highly prevalent isolates belong to international clonal lineage (ICL)-II. These results indicate that the wide horizontal and clonal spread of MDR Acinetobacter spp. isolates harbouring multiple β-lactamase genes has become a serious problem in northeastern China.