Environmental Staphylococcus aureus contamination in a Tunisian hospital. (1st November 2016)

• Record Type: Journal Article
• Authors: Gharsa, Haythem; Dziri, Raoudha; Klibi, Naouel; Chairat, Sarra; Lozano, Carmen; Torres, Carmen; Bellaaj, Ridha; Slama, Karim Ben
• Is Part Of: Journal of chemotherapy. Volume 28:Number 6(2016:Dec.)
• Page Start: 506
• Page End: 509
• Publication Date: 2016-11-01
• Subjects: Staphylococcus aureus -- Methicillin-resistance -- TSST -- Environmental hospital -- Tunisia; Chemotherapy -- Periodicals; Drug Therapy -- Periodicals; 615.58
• DOI: 10.1179/1973947815Y.0000000036
• Languages: English
• ISSNs: 1120-009X

Abstract

Abstract : One hundred hospital environment samples were obtained in 2012 in a Tunisian hospital and tested for Staphylococcus aureus recovery. Antimicrobial resistance profile and virulence gene content were determined. Multilocus-sequence-typing (MLST), spa -typing, agr -typing and Sma I-pulsed-field gel electrophoresis (PFGE) were performed. Two methicillin-resistant S. aureus (MRSA) isolates typed as: ST247-t052-SCC mec I– agr I were recovered from the intensive care unit (ICU). Ten samples contained methicillin-susceptible S. aureus (MSSA) and these samples were collected in different services, highlighting the presence of the tst gene encoding the toxic shock syndrome toxin as well as the luk ED, hla, hlb, hld and hlg v virulence genes in some of the isolates. In conclusion, we have shown that the hospital environment could be a reservoir contributing to dissemination of virulent S. aureus and MRSA.