Use of panel testing for detection of antinuclear antibody in a resource-limited setting: an appraisal. Issue 8 (16th November 2016)

• Record Type: Journal Article
• Authors: Minz, Ranjana Walker; Kumar, Yashwant; Saikia, Biman; Anand, Shashi; Varma, Subhash; Singh, Surjit
• Is Part Of: Postgraduate medicine. Volume 128:Issue 8(2016)
• Page Start: 869
• Page End: 874
• Publication Date: 2016-11-16
• Subjects: CTD -- ANA -- LIA -- Indirect immunofluorescence -- dsDNA ELISA; Medicine -- Periodicals; Medicine -- Periodicals; Medicine; Periodicals; 610.5
• DOI: 10.1080/00325481.2016.1220808
• Languages: English
• ISSNs: 0032-5481

Abstract

ABSTRACT: Objectives : Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty. Methods : To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD. Results : Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm. Conclusion : Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening.