Myelin‐targeted, texaphyrin‐based multimodal imaging agent for magnetic resonance and optical imaging. (6th September 2016)

• Record Type: Journal Article
• Authors: Vithanarachchi, Sashiprabha M.; Foley, Casey D.; Trimpin, Sarah; Ewing, James R.; Ali, Meser M.; Allen, Matthew J.
• Is Part Of: Contrast media & molecular imaging. Volume 11:Number 6(2016:Nov./Dec.)
• Page Start: 492
• Page End: 505
• Publication Date: 2016-09-06
• Subjects: contrast agents -- gadolinium -- MRI -- multimodal probes -- myelin -- optical imaging -- paramagnetic agents -- texaphyrin; Diagnostic imaging -- Periodicals; Magnetic resonance imaging -- Periodicals; Contrast media (Diagnostic imaging) -- Periodicals; Contrast Media -- Periodicals; Diagnostic Imaging -- Periodicals; Substances de contraste -- Périodiques; Diagnostics moléculaires -- Périodiques; Imagerie médicale; Substance de contraste; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.0754
• DOI: 10.1002/cmmi.1711
• Languages: English
• ISSNs: 1555-4309

Abstract

Abstract : Reliable methods of imaging myelin are essential to investigate the causes of demyelination and to study drugs that promote remyelination. Myelin‐specific compounds can be developed into imaging probes to detect myelin with various imaging techniques. The development of multimodal myelin‐specific imaging probes enables the use of orthogonal imaging techniques to accurately visualize myelin content and validate experimental results. Here, we describe the synthesis and application of multimodal myelin‐specific imaging agents for light microscopy and magnetic resonance imaging. The imaging agents were synthesized by incorporating the structural features of luxol fast blue MBS, a myelin‐specific histological stain, into texaphyrins coordinated to Gd III . These new complexes demonstrated absorption of visible light, emission of near‐IR light, and relaxivity values greater than clinically approved contrast agents for magnetic resonance imaging. These properties enable the use of optical imaging and magnetic resonance imaging for visualization of myelin. We performed section‐ and en block ‐staining of ex vivo mouse brains to investigate the specificity for myelin of the new compounds. Images obtained from light microscopy and magnetic resonance imaging demonstrate that our complexes are retained in white matter structures and enable detection of myelin. Copyright © 2016 John Wiley & Sons, Ltd. Abstract : Visualization of myelinated structures is vital for research related to neuropathology and aging. We synthesized multimodal myelin‐specific imaging agents to detect myelinated structures. Optical microscopy and magnetic resonance imaging were performed on the same ex vivo tissue samples to detect myelinated structures. The new imaging agents have potential to provide insight into neuropathology by enabling accurate detection of myelin and validation of results with orthogonal imaging techniques.