Cell Surface Nano‐modulation for Non‐invasive in vivo Near‐IR Stem Cell Monitoring. (29th December 2016)

• Record Type: Journal Article
• Authors: Shin, Woo Jung; Shin, Seung Won; Yuk, Ji Soo; Amornkitbamrung, Lunjakorn; Jang, Min Su; Song, In Hyun; Choi, Soon Won; Kang, Insung; Lee, Jin Young; Bae, Hojae; Kang, Kyung‐Sun; Um, Soong Ho
• Is Part Of: ChemMedChem. Volume 12:Number 1(2017)
• Page Start: 28
• Page End: 32
• Publication Date: 2016-12-29
• Subjects: in vivo monitoring -- nanotechnology -- near-IR -- stem cells -- surface modulation; Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1002/cmdc.201600428
• Languages: English
• ISSNs: 1860-7179

Abstract

Abstract: A stem cell tracking system is in high demand for the determination of cell destinations and for the validation of cell therapeutic efficacy in regenerative transplantation. To date, near‐infrared (NIR) imaging technology has received considerable attention in cell behavior monitoring, owing to its patient compatibility, easy accessibility and cost effectiveness. Conventionally, in vivo cell tracking has been visualized by direct in‐cell staining with NIR, where it may be achieved by complicated genetic engineering. Such genetic amendment techniques have suffered from serious challenges, which can destroy a cell's metabolism and can accidentally incur unexpected carcinoma. Herein we demonstrate a novel cell nano‐modulation method for noninvasive stem cell monitoring. It is simply achieved by conjugating stem cells with lipid‐supported, NIR‐tagged, polymeric nanoparticles. These engineered cells, which are designated as NIR‐labeled light‐emitting stem cells (LESCs), maintain their biochemical functionality (i.e., differentiation, quantum efficacy, etc.) even after conjugation. LESCs were used for in situ stem cell monitoring at inoculation sites. It is speculated that the LESC technique could provide a new preparative methodology for in vivo cell tracking in advanced diagnostic medicine, where cell behavior is a critical issue. Abstract : Under close watch : Engineering of light‐emitting lipid‐supported poly(lactic‐co‐glycolic acid) nanoparticles (LENPs) to human umbilical‐cord‐derived mesenchymal stem cells (hUCB‐MSCs) results in light‐emitting stem cells, which are newly designated as LESCs. LESCs were monitored in vivo under near‐infrared after being injected in vivo.