Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR. (30th November 2016)

• Record Type: Journal Article
• Authors: Luo, Yu; Bolt, Hannah L.; Eggimann, Gabriela A.; McAuley, Danny F.; McMullan, Ronan; Curran, Tanya; Zhou, Mei; Jahoda, Professor Colin A. B.; Cobb, Steven L.; Lundy, Fionnuala T.
• Is Part Of: Chembiochem. Volume 18:Number 1(2017)
• Page Start: 111
• Page End: 118
• Publication Date: 2016-11-30
• Subjects: antibacterial -- antibiotics -- antiproliferation -- cross kingdom -- peptoid -- quantification; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201600381
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide‐mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms. Selected peptoids showed significant bactericidal and fungicidal activity against the polymicrobial biofilms. This coupled with low cytotoxicity suggests that peptoids could offer a new option for the treatment of clinically relevant polymicrobial infections. Abstract : Peptoids can mimic host‐defence antimicrobial peptides but have increased resistance to proteases. We constructed a library of such peptoids and showed that they reduce the cell numbers of C. albicans, S. aureus and E. coli in polymicrobial biofilms, as quantified by a novel PMA‐qPCR assay.