Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery. Issue 2 (15th December 2016)

• Record Type: Journal Article
• Authors: Bhat, Satish S.; Revankar, Vidyanand K.; Khan, Ayesha; Pinjari, Rahul V.; Necas, Marek
• Is Part Of: Chemistry, an Asian journal. Volume 12:Issue 2(2017)
• Page Start: 254
• Page End: 264
• Publication Date: 2016-12-15
• Subjects: DNA -- electrochemistry -- luminescence -- photochemistry -- ruthenium; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201601515
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)2 (tpt‐phen)]Cl2 (1 ; bpy=2, 2′‐bipyridine, tpt‐phen=triptycenyl‐1, 10‐phenanthroline) and [Ru(phen)2 (tpt‐phen)]Cl2 (2 ; phen=1, 10‐phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities. Abstract : Label‐free DNA carriers : Two new ruthenium(II) polypyridyl complexes have been developed as nonviral vectors for DNA delivery. These complexes efficiently condense DNA into globular nanoparticles, and thus, can be used as potential DNA carriers (see figure).