Novel genes in brain tissues of EAE-induced normal and obese mice: Upregulation of metal ion-binding protein genes in obese-EAE mice. (20th February 2017)

• Record Type: Journal Article
• Authors: Hasan, Mahbub; Seo, Ji-Eun; Rahaman, Khandoker Asiqur; Min, Hophil; Kim, Ki Hun; Park, Ju-Hyung; Sung, Changmin; Son, Junghyun; Kang, Min-Jung; Jung, Byung Hwa; Park, Won Sang; Kwon, Oh-Seung
• Is Part Of: Neuroscience. Volume 343(2017)
• Page Start: 322
• Page End: 336
• Publication Date: 2017-02-20
• Subjects: CNS central nervous system -- DEGs differentially expressed genes -- EAE experimental autoimmune encephalomyelitis -- GO gene ontology -- HFD high-fat diet -- MHC major histocompatibility complex -- MOG35-55 myelin oligodendrocyte glycoprotein 35–55 -- MS multiple sclerosis -- ND normal diet -- RT-PCR reverse-transcriptase polymerase chain reaction -- Th T-helper cells; experimental autoimmune encephalomyelitis -- gene microarray -- diet-induced obesity -- metal ion-binding protein genes; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.12.002
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: An aggravated EAE was observed in HFD-induced obese mice compared with ND-mice. Significantly upregulated genes in EAE were related to immune response, antigen presentation, complement activation, etc. C4b, Psmb8, Ly86, Ms4a6d, Cul9, and Mast2 are novel genes upregulated in both ND- and HFD-EAE mice. Cul9 and Mast2 genes of metal ion-binding proteins were upregulated in HFD-EAE mice than in ND-EAE mice. Abstract: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory autoimmune disease of the central nervous system resulting from degeneration of the myelin sheath. This study is aimed to identify differentially expressed genes (DEGs) in the brain of EAE-induced normal diet (ND) mice and high-fat diet (HFD)-induced obese mice, and to identify novel genes responsible for elucidating the mechanism of the disease. Purified mRNA samples from the brain tissue were analyzed for gene microarray and validated by real-time RT-PCR. DEGs were identified if significant changes greater than 1.5-fold or less than 0.66-fold were observed ( p < 0.05). Pathway construction and functional categorization were performed using the Kyoto encyclopedia of genes and genomes pathways and gene ontology (GO) analysis. HFD-EAE mice showed more severe disease symptoms than ND-EAE mice. From GO study, fold changes of HFD-EAE to ND-EAE genes indicated that the genes were significantly associated to the pathways related with the immune response, antigen presentation, and complement activation. The genes related with metal ion-binding proteins were upregulated in HFD-EAE and ND-EAE mice. Upregulation of Cul9, Mast2, and C4b expression is significantly higher in HFD-EAE mice than ND-EAE mice. Cul9, Mast2, C4b, Psmb8, Ly86, and Ms4a6d were significantly upregulated in both ND- and HFD-EAE mice. Fcgr4, S3-12, Gca, and Zdhhc4 were upregulated only in ND-EAE, and Xlr4b was upregulated only in HFD-EAE mice. And significant upregulated genes of metal ion-binding proteins ( Cul9 and Mast2 ) were observed in HFD-EAE mice.