Punica granatum L. mesocarp suppresses advanced glycation end products (AGEs)- and H2O2-induced oxidative stress and pro-inflammatory biomarkers. (February 2017)

• Record Type: Journal Article
• Authors: Ramlagan, Piteesha; Rondeau, Philippe; Planesse, Cynthia; Neergheen-Bhujun, Vidushi S.; Fawdar, Shameem; Bourdon, Emmanuel; Bahorun, Theeshan
• Is Part Of: Journal of functional foods. Volume 29(2017)
• Page Start: 115
• Page End: 126
• Publication Date: 2017-02
• Subjects: AAPH 2, 2′-azobis(2-amidinopropane) dihydrochloride -- ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) -- AGEs advanced glycation end products -- AOPP advanced oxidation protein product -- BSAG0 native bovine serum albumin -- BSAMGO BSA glycated with methylglyoxal -- CAT catalase -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- FRAP ferric reducing antioxidant power -- GPx glutathione peroxidase -- IL-6 interleukin 6 -- iNOS inducible nitric oxide synthase -- MCP-1 monocyte chemoattractant protein 1 -- NFκB nuclear transcription factor kappa B -- NOX1 nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 -- ORAC oxygen radical absorbance capacity -- PME pomegranate mesocarp extract -- ROS reactive oxygen species -- SOD superoxide dismutase -- TNF-α tumour necrosis factor alpha; AGEs -- Antioxidants -- Oxidative stress -- Pomegranate -- Preadipocytes; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.12.007
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Effects of PME on oxidative stress and inflammation in 3T3-L1 were assessed. PME decreased ROS production by increasing activities of antioxidant enzymes. PME down regulated NOX1 expression and inhibited oxidative modification of proteins. PME exerted anti-inflammatory effect by decreasing NFκB, iNOS and IL-6 expressions. PME down-regulated mRNA and protein expressions of AGEs receptor CD36. Abstract: The protective potential of pomegranate ( Punica granatum L.) mesocarp extract (PME) on a cell model mimicking diabetes-like oxidative stress exposed to advanced glycation end products (AGEs) and hydrogen peroxide (H2 O2 ) was investigated. The polyphenolic antioxidant rich PME afforded protection to erythrocytes from AAPH radical-induced lysis and significantly reduced (P < 0.001) ROS production in 3T3-L1 preadipocytes by preventing AGEs-modulated decrease in intrinsic antioxidant enzymatic activities and by reducing NOX1 expression. PME significantly inhibited protein carbonyl and AOPP levels following their increase in the presence of AGEs and H2 O2 (P < 0.05). It exerted an anti-inflammatory effect on preadipocytes by suppressing IL-6 secretion. PME down-regulated the over-expression of pro-inflammatory mediators NFκB, iNOS and IL-6 post-AGEs treatment and concomitantly down-regulated the expression of AGEs receptor CD36 in the presence of AGEs. PME decreases oxidative stress and inflammation at adipose tissue level with the propensity to mitigate obesity-related disorders such as insulin resistance and type II diabetes.