Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells. (February 2017)

• Record Type: Journal Article
• Authors: Lopez, Sergio; Montserrat-de la Paz, Sergio; Lucas, Ricardo; Bermudez, Beatriz; Abia, Rocio; Morales, Juan C.; Muriana, Francisco J.G.
• Is Part Of: Journal of functional foods. Volume 29(2017)
• Page Start: 238
• Page End: 247
• Publication Date: 2017-02
• Subjects: AUC area-under-the-curve -- CCL2 chemokine (CC) motif ligand 2 -- DAPI diamino-fenilindol -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- GCLC glutamate-cysteine ligase catalytic subunit -- GPx1 glutathione peroxidase 1 -- GSH glutathione -- hECs human endothelial cells -- HO-1 heme oxygenase-1 -- HPRT hypoxantine-guanine phosphoribosyltransferase -- HTyr hydroxytyrosol -- HTyr-GLU hydroxytyrosol glucuronate metabolites -- HTyr-O-GLU hydroxytyrosol orto-glucuronate metabolites -- HTyr-SUL hydroxytyrosol sulfate metabolites -- ICAM-1 intercellular adhesion molecule 1 -- MPO myeloperoxidase -- MTT methylthiazolyldiphenyl-tretrazolium bromide -- NF-κB nuclear factor kappa B -- PTGS2 prostaglandin-endoperoxidase synthase 2 -- ROS reactive oxygen species -- TBDMS tert-butyldimethylsilyl -- TNF-α tumour necrosis factor alpha -- TPA 12-O-tetradecanoylphorbol-13-acetate -- VCAM-1 vascular adhesion molecule 1; Hydroxytyrosol -- Metabolites -- Inflammation -- Endothelial cells -- Human -- Mice; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.12.033
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: HTyr sulfate metabolites protect antioxidant defenses from TNF-α in endothelial cells. HTyr (sulfate > glucuronate) metabolites also protect from the inflammatory response. HTyr (sulfate > glucuronate) metabolites decrease adhesiveness of endothelial cells. HTyr sulfate metabolites suppress acute and chronic inflammation in mice. Abstract: The effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (C C) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12- O -tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.