A metabolic profiling approach to an Italian sage leaf extract (SoA541) defines its antioxidant and anti-acetylcholinesterase properties. (February 2017)

• Record Type: Journal Article
• Authors: Pacifico, Severina; Piccolella, Simona; Lettieri, Annamaria; Nocera, Paola; Bollino, Flavia; Catauro, Michelina
• Is Part Of: Journal of functional foods. Volume 29(2017)
• Page Start: 1
• Page End: 9
• Publication Date: 2017-02
• Subjects: LC-MS/MS liquid chromatography mass/mass spectrometry -- CNS central nervous system -- AD Alzheimer's Disease -- AChE acetylcholinesterase -- AChEI acetylcholinesterase inhibitors -- UAM ultrasound assisted maceration -- LLE liquid liquid extraction -- Q quercetin -- RA rosmarinic acid -- CA carnosic acid -- MTT 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide -- TBARS ThioBarbituric Acid Reactive Species -- DCFH-DA 2′, 7′-dichlorofluorescein diacetate -- CAA cellular antioxidant activity -- ABAP 2, 2′-azobis (2-methylpropionamidine) dihydrochloride -- MDA malondialdehyde -- RAI redox activity inhibition -- AUC area under curve -- ROS reactive oxygen species -- CAD collision activated dissociation; Caffeic acid glucoside (PubChem CID: 5281761) -- 6-O-Feruloylglucose (PubChem CID: 71307318) -- Luteolin-7-glucoside (PubChem CID: 5280637) -- Apigenin-7-glucoside (PubChem CID: 5280704) -- Rosmarinic acid (PubChem CID: 5281792) -- Rosmanol (PubChem CID: 13966122) -- Epirosmanol (PubChem CID: 23243694) -- Rosmadial (PubChem CID: 15801061) -- Carnosol (PubChem CID: 442009) -- Carnosic acid (PubChem CID: 65126); Salvia officinalis -- LC-ESI-MS/MS -- Phenols -- Abietane diterpenes -- Cytotoxicity -- Antioxidant activity; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.11.031
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: SoA541 was a phenolic enriched extract from leaves of cultivated sage plants. It was quali- and quantitatively analyzed through LC-ESI-MS/MS techniques. SoA541 originally contained lignin oligomers, beyond other known constituents. The extract showed anti-lipoperoxidant, antioxidant and anti-AChE properties. Abstract: LC-MS/MS techniques were applied to analyze, quali- and quantitatively, a phenol extract from leaves of Salvia officinalis, cultivated in the Campania Region (Italy). It accounted for ∼50% of abietane diterpenes, ∼40% of phenylpropanoid constituents, and 10% flavonoids, and so called SoA541. Lignin oligomers were detected for the first time as sage constituents. SoA541 exerted anti-lipoperoxidative and antioxidant properties at dose levels <125 μg/mL on SH-SY5Y cells, and its ability to inhibit the AChE enzyme was far greater than donepezil. The anti-AChE effectiveness was dose-dependent up to a maximum (62.5 μg/mL), beyond which the effect decreased. The data obtained encourage further research aimed at evaluating a potential use of the "Campania" sage in the prevention of CNS diseases in animal models.