In vivo and in vitro anti-obesity activities of dihydropyranocoumarins derivatives from Peucedanum japonicum Thunb. (February 2017)

• Record Type: Journal Article
• Authors: Taira, Naoyuki; Nugara, Ruwani N.; Inafuku, Masashi; Takara, Kensaku; Ogi, Takayuki; Ichiba, Toshio; Iwasaki, Hironori; Okabe, Takafumi; Oku, Hirosuke
• Is Part Of: Journal of functional foods. Volume 29(2017)
• Page Start: 19
• Page End: 28
• Publication Date: 2017-02
• Subjects: Acaca acetyl-CoA carboxylase 1 -- AMD (+)-cis-3′-acetoxy-4′-(2-methylbutyroyloxy)-3′-4′-dihydroseselin -- BCS bovine calf serum -- CC coumarin concentrate -- Cebpa CCAAT-enhancer-binding protein, alpha -- cDIVK cis-3′, 4′-diisovalerylkhellactone -- Cpt1a carnitine palmitoyltransferase 1a -- DMEM Dulbecco's modified Eagle's medium -- DMSO dimethyl sulfoxide -- Fasn fatty acid synthase -- FBS fetal bovine serum -- HFD high-fat diet -- HIV human immunodeficiency virus -- HSD honestly significant difference -- HPLC high-performance liquid chromatography -- I.D. internal diameter -- ISD isosamidin -- SA1/1 SKD/AMD (1/1) -- Slc2a4 glucose transporter type 4 -- SKD suksdorfin -- PBS phosphate buffered saline -- PCIII peucedanocoumarin III -- Pgp P-glycoprotein -- PJT Peucedanum japonicum Thunb -- PPAR peroxisome proliferator-activated receptor -- Ppara PPARα -- Pparg PPARγ -- PTX pteryxin -- qPCR quantitative real-time polymerase chain reaction -- Scd1 stearoyl CoA desaturase 1 -- SEM standard error of the mean -- Srebf1 sterol regulatory element-binding protein 1 -- TG triglyceride -- Ucp2 uncoupling protein 2 -- VIS visnadine -- WAT white adipose tissue; Pteryxin (PubChem CID: 5281425) -- Peucedanocoumarin III (PubChem CID: 1032882) -- Isosamidin (PubChem CID: 442133) -- Suksdorfin (PubChem CID: 72414) -- Visnadine (PubChem CID: 442151); Peucedanum japonicum Thunb -- Dihydropyranocoumarin -- Anti-obesity -- 3T3-L1 adipocyte; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.11.030
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: We identified two varieties of Peucedanum japonicum Thunb (PJT). Dietary coumarin concentrates from two varieties of PJT inhibited obesity. Several coumarins from PJT exhibited distinct in vitro anti-obesity activities. Abstract: Several types of coumarins have been isolated from Peucedanum japonicum Thunb (PJT) and we previously reported the anti-obesity activity of pteryxin (PTX), as an active compound of PJT. However, during our research, we identified another variety of PJT, which does not contain any PTX. Here, we prepared concentrates from the two varieties of PJT and demonstrated that dietary PJT concentrates both with and without PTX significantly decreased the relative weights of white adipose tissue (WAT) and mRNA levels of lipogenic genes in epididymal WAT. Furthermore, we compared the inhibitory effects of dihydropyranocoumarins of PJT on lipid accumulation of differentiated 3T3-L1 adipocytes. All of the dihydropyranocoumarins used in this study significantly inhibited lipid accumulation and lipogenic-related gene expressions in 3T3-L1 adipocytes. However, the magnitudes of the effects were distinctly different. The present study demonstrated that several dihydropyranocoumarins in PJT possess anti-obesity activities, which mainly act by inhibiting lipogenesis in adipocytes.