Pros and cons of direct genotyping on tuberculosis clinical samples. (February 2017)

• Record Type: Journal Article
• Authors: Sadegh, Hamidreza; Kargarpour Kamakoli, Mansour; Farmanfarmaei, Ghazaleh; Masoumi, Morteza; Abdolrahimi, Farid; Fateh, Abolfazl; Ebrahimzadeh, Nayereh; Rahimi Jamnani, Fatemeh; Vaziri, Farzam; Siadat, Seyed Davar
• Is Part Of: Microbial pathogenesis. Volume 103(2017)
• Page Start: 135
• Page End: 138
• Publication Date: 2017-02
• Subjects: Prompt genotyping -- Mycobacterium tuberculosis -- 24 loci MIRU-VNTR -- Mixed infections; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.12.025
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Objective: Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). Methods: We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients. Results: There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load ( P = 0.001). VNTR loci were amplified successfully for 41.5% of the clinical samples (19–24 loci), 32.6% (13–18 loci), 23.7% (7–12 loci) and 2.2% (1–6 loci). Loci of 2401, 577, 2996 and 154 had the highest power to show the mixed strains infection in clinical samples. Conclusions: Direct MIRU-VNTR is partially successful in complete genotyping of M. tuberculosis strains. On the other hand, detection of polyclonal infection is undoubtedly reliable based on the direct MIRU-VNTR. Highlights: Direct MIRU-VNTR is partially successful in complete genotyping of M. tuberculosis strains. Detection of polyclonal infection undoubtedly reliable based on the direct MIRU-VNTR. There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load ( P = 0.001).