Evaluation of DNA-damaging potential of bisphenol A and its selected analogs in human peripheral blood mononuclear cells (in vitro study). (February 2017)

• Record Type: Journal Article
• Authors: Mokra, Katarzyna; Kuźmińska-Surowaniec, Agnieszka; Woźniak, Katarzyna; Michałowicz, Jaromir
• Is Part Of: Food and chemical toxicology. Volume 100(2017)
• Page Start: 62
• Page End: 69
• Publication Date: 2017-02
• Subjects: Bisphenol A -- Bisphenol A analogs -- Comet assay -- DNA damage -- DNA repair -- PBMCs; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.12.003
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: In the present study, we have investigated DNA-damaging potential of BPA and its analogs, i.e. bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) in human peripheral blood mononuclear cells (PBMCs) using the alkaline and neutral versions of the comet assay, which allowed to evaluate DNA single strand-breaks (SSBs) and double strand-breaks (DSBs). The use of the alkaline version of comet assay made also possible to analyze the kinetics of DNA repair in PBMCs after exposure of the cells to BPA or its analogs. We have observed an increase in DNA damage in PBMCs treated with BPA or its analogs in the concentrations ranging from 0.01 to 10 μg/ml after 1 and 4 h incubation. It was noted that bisphenols studied caused DNA damage mainly via SSBs, while DNA fragmentation via double DSBs was low. The strongest changes in DNA damage were provoked by BPA and particularly BPAF, which were capable of inducing SSBs even at 0.01 μg/ml, while BPS caused the lowest changes (only at 10 μg/ml). We have also observed that PBMCs significantly repaired bisphenols-induced DNA damage but they were unable (excluding cells treated with BPS) to repair totally DNA breaks. Highlights: Effect of BPA and its selected analogs on DNA damage and repair in PBMCs was studied. BPAF induced the strongest DNA single strand-breaks and double strand-breaks in PBMCs. BPS, which is main substituent of bisphenol A caused much lower DNA damage than BPA. PBMCs significantly repaired bisphenols-induced DNA damage. BPA and BPAF at low concentration of 0.01 μg/ml were able to induce single strand-breaks.