Enhanced cytotoxic and apoptosis inducing activity of lycopene oxidation products in different cancer cell lines. (November 2016)

• Record Type: Journal Article
• Authors: Arathi, Bangalore Prabhashankar; Sowmya, Poorigali Raghavendra-Rao; Kuriakose, Gini Chempakathinal; Vijay, Kariyappa; Baskaran, Vallikannan; Jayabaskaran, Chelliah; Lakshminarayana, Rangaswamy
• Is Part Of: Food and chemical toxicology. Volume 97(2016)
• Page Start: 265
• Page End: 276
• Publication Date: 2016-11
• Subjects: Lycopene oxidation products -- Cytotoxicity -- Apoptosis -- Reactive oxygen species -- Free radicals -- UPLC-MS; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.09.016
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Currently, upon understanding the metabolomics of carotenoids, it is important to address the key role of carotenoid derived products. In this regard, aim of the study was to elucidate and explore the role of lycopene (LYC) oxidative products generated through autoxidation (AOL) or chemical (KMnO4 ) oxidation (COL) against proliferation of selected cancer cells. Preliminary, we investigated the effect of LYC on cell viability of various cancer cell lines (PC-3, MCF-7, A431, HepG2, HeLa and A549). Based on the results of LYC treatment on cell cytotoxicity levels, MCF-7, PC-3 and HeLa cell lines were further tested with AOL and COL products. The decreased cell viability with depleted GSH and increased MDA levels were observed when treated with COL products than control, LYC and AOL. In addition, COL products increased ROS levels and percent apoptosis. The typical morphological changes and nuclear condensations showed that COL products have anti-proliferation and apoptosis inducing activity. Based on results, we hypothesized that ROS generation by LYC oxidation products may be one of intermediate step involved in apoptosis. The redox status and therapeutic approach of COL products in modulating ROS and induction of apoptosis in cancer cells were reported for the first time, to our knowledge. To conclude, COL products involves in cancer growth inhibition efficiently than intact LYC and AOL. Hence, there is a great potential for synthesizing or producing such carotenoid oxidation products to augment cancer complication. Graphical abstract: Highlights: Lycopene doses influences variably in different cancer cell lines. Chemically induced lycopene oxidised products (COL) were characterized by MS (APCI) + mode. COL products decreased cell viability with depleted GSH and increased MDA levels in cancer cells. COL products induced apoptosis with intermediate ROS generation in MCF-7, PC-3 and HeLa cancer cell lines.