Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance. (14th September 2015)

• Record Type: Journal Article
• Authors: Liu, Jun; Guo, Ting; Wang, Dong; Xu, Jiahui; Ying, Hanjie
• Is Part Of: Biotechnology and applied biochemistry. Volume 63:Number 5(2016:Sep./Oct.)
• Page Start: 727
• Page End: 733
• Publication Date: 2015-09-14
• Subjects: Clostridium beijerinckii -- ferulic acid -- fermentation -- high‐throughput screening -- non‐detoxified hemicellulosic hydrolysate; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1418
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: A mutant strain of Clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high‐throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non‐ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13 g/L of butanol using non‐detoxified hemicellulosic hydrolysate from diluted sulfuric acid‐treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non‐detoxified SAHHC for butanol production.