Stimulation of the activity of a novel tannase produced in white‐rot fungi Phellinus pini, Fomes fomentarius, and Tyromyces pubescens by medium supplementation. (9th September 2015)

• Record Type: Journal Article
• Authors: Prendecka, Monika; Jaszek, Magdalena; Grąz, Marcin; Głuszak, Natalia; Małysz, Katarzyna; Nowak, Agata; Żuchowski, Jerzy; Małecka‐Massalska, Teresa
• Is Part Of: Biotechnology and applied biochemistry. Volume 63:Number 5(2016:Sep./Oct.)
• Page Start: 652
• Page End: 658
• Publication Date: 2015-09-09
• Subjects: tannase -- white‐rot fungi -- gallic acid -- capillary electrophoresis, tannic acid -- rapeseed meal; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1413
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: In recent years, tannase has gained increasing interest mainly because of its potential applications. One of the most important functions of tannic acid (TA) hydrolase is the release of gallic acid (GA) from complex tannins. The aim of the study was to determine the dynamic changes in tannase activity depending on the carbon source in the culture medium. An extracellular and intracellular tannase activity analysis was carried out with the use of spectrophotometric analysis and confirmed by capillary electrophoresis in cultures of white‐rot fungi: Phellinus pini, Fomes fomentarius, and Tyromyces pubescens . The inducible potential of TA and rapeseed meal on the activity of tannin acyl hydrolase was confirmed during 14 days of culturing. Different effects of the tested compounds on stimulation of tannase activity in selected fungal strains have been demonstrated. We concluded that rapeseed meal was the best inducer of tannase activity in the case of P. pini . However, the highest concentrations of GA were observed after stimulation by the TA in the cultures of F. fomentarius and T. pubescens .