Dendrimer-functionalized LAPONITE® nanodisks loaded with gadolinium for T1-weighted MR imaging applications. Issue 97 (5th October 2016)

• Record Type: Journal Article
• Authors: Mustafa, Rania; Zhou, Benqing; Yang, Jia; Zheng, Linfeng; Zhang, Guixiang; Shi, Xiangyang
• Is Part Of: RSC advances. Volume 6:Issue 97(2016)
• Page Start: 95112
• Page End: 95119
• Publication Date: 2016-10-05
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra18718h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Dendrimer-functionalized LAPONITE® nanodisks loaded with gadolinium can be used as an efficient contrast agent for different MR imaging applications. Abstract : In this report, we present a facile approach to forming dendrimer-functionalized LAPONITE® (LAP) nanodisks loaded with gadolinium (Gd) for in vitro and in vivo T 1 -weighted MR imaging applications. In this work, LAP nanodisks were sequentially modified with silane coupling agents, succinic anhydride to have abundant carboxyl groups on their surface, and amine-terminated poly(amidoamine) (PAMAM) dendrimers of generation 2 (G2). The dendrimer-modified LAP nanodisks were then conjugated with gadolinium (Gd) chelator diethylenetriaminepentaacetic acid (DTPA), followed by Gd(iii ) chelation to form the LM–G2–DTPA (Gd) nanocomplexes. The designed LM–G2–DTPA(Gd) nanocomplexes were characterized via different techniques. Cell viability assay shows that the formed LM–G2–DTPA(Gd) nanodisks are non-cytotoxic in the given concentration range. With a high r 1 relaxivity (2.05 mM −1 s −1 ), the LM–G2–DTPA(Gd) nanocomplexes are able to be used as an efficient contrast agent for T 1 -weighted MR imaging of cancer cells in vitro and animal organ/tumor model in vivo . The designed LM–G2–DTPA(Gd) nanocomplexes may hold a great promise to be used as a versatile nanoplatform for MR imaging of different biological systems.