BCFA suppresses LPS induced IL-8 mRNA expression in human intestinal epithelial cells. (January 2017)

• Record Type: Journal Article
• Authors: Yan, Y.; Wang, Z.; Greenwald, J.; Kothapalli, K.S.D.; Park, H.G.; Liu, R.; Mendralla, E.; Lawrence, P.; Wang, X.; Brenna, J.T.
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 116(2017:Jan.)
• Page Start: 27
• Page End: 31
• Publication Date: 2017-01
• Subjects: Caco-2 cells -- BCFA -- IL-8 -- Inflammation; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2016.12.001
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: Branched chain fatty acids (BCFA) are components of common food fats and are major constituents of the normal term human newborn GI tract. Polyunsaturated fatty acids (PUFA) have been suggested to reduce the risk and development of inflammatory bowel diseases (IBD); however, little is known about the influence of BCFA on inflammation. We investigated the effect of BCFA on interleukin (IL)-8 and NF-κB production in a human intestinal epithelial cell line (Caco-2). Cells were pre-treated with specific BCFA, or DHA, or EPA, and then activated with lipopolysaccharide (LPS). Both anteiso- and iso- BCFA reduce IL-8. Anteiso- BCFA more effectively suppressed IL-8 than iso- BCFA in LPS stimulated Caco-2 cells. However BCFA in general were less effective than DHA or EPA. Activated BCFA-treated cells expressed less of the cell surface Toll-like receptor 4 (TLR-4) compared to controls. These are the first data to show the reduction of pro-inflammatory markers in human cells mediated by BCFA. Highlights: Branched chain fatty acids are associated with lower inflammatory markers and disease in vivo. Membrane lipids readily incorporate high levels of BCFA. Pro-inflammatory IL-8 mRNA and the downstream signaling NF-κB decrease in LPA-stimulated cells. Anteiso-BCFA more actively lower inflammatory markers than iso-BCFA.