Antibacterial activity of fractions from three Chumash medicinal plant extracts and in vitro inhibition of the enzyme enoyl reductase by the flavonoid jaceosidin. Issue 6 (19th March 2017)

• Record Type: Journal Article
• Authors: Allison, Brittany J.; Allenby, Mark C.; Bryant, Shane S.; Min, Jae Eun; Hieromnimon, Mark; Joyner, P. Matthew
• Is Part Of: Natural product research. Volume 31:Issue 6(2017)
• Page Start: 707
• Page End: 712
• Publication Date: 2017-03-19
• Subjects: Artemisia californica -- enoyl reductase -- jaceosidin -- native American plants -- antimicrobial -- flavonoids; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2016.1217201
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: We have investigated the in vitro antibacterial bioactivity of dichloromethane-soluble fractions of Artemisia californica, Trichostema lanatum, Salvia apiana, Sambucus nigra ssp. cerulea and Quercus agrifolia Née against a Δ tolC mutant strain of Escherichia coli . These plants are traditional medicinal plants of the Chumash American Indians of Southern California. Bioassay-guided fractionation led to the isolation of three flavonoid compounds from A. californica : jaceosidin (1 ), jaceidin (2 ), and chrysoplenol B (3 ). Compounds1 and2 exhibited antibacterial activity against E. coli Δ tolC in liquid cultures. The in vitro activity of1 against the enoyl reductase enzyme (FabI) was measured using a spectrophotometric assay and found to completely inhibit FabI activity at a concentration of 100 μM. However, comparison of minimum inhibitory concentration values for1 –3 against E. coli Δ tolC and an equivalent strain containing a plasmid constitutively expressing fabI did not reveal any selectivity for FabI in vivo . Abstract :