A novel fluorescent indicator displacement assay for sensing the anticancer drug gefitinib. Issue 3 (4th March 2017)

• Record Type: Journal Article
• Authors: Xi, Yun-Yun; Tang, Qing; Huang, Ying; Tao, Zhu; Xue, Sai-Feng; Zhou, Qingdi; Wei, Gang
• Is Part Of: Supramolecular chemistry. Volume 29:Issue 3(2017)
• Page Start: 229
• Page End: 235
• Publication Date: 2017-03-04
• Subjects: Cucurbit[8]uril -- cell imaging -- fluorescent indicator displacement assay -- gefitinib; Macromolecules -- Periodicals; Supramolecular chemistry -- Periodicals; 547.005
• DOI: 10.1080/10610278.2016.1202413
• Languages: English
• ISSNs: 1061-0278

Abstract

Abstract: Fluorescent indicator displacement assays have become popular for converting synthetic receptors into optical sensors. We have now shown that a 1:2 host–guest complex between cucurbit[8]uril (Q[8]) and proflavine (PF) can be used as a fluorescent indicator for sensing the anticancer drug gefitinib. The 2PF@Q[8] complex can be used to detect gefitinib with high selectivity using fluorescence spectrometry with a detection limit of 6.63 × 10 −8 mol −L . The proposed sensing mechanism was investigated using 1 H nuclear magnetic resonance spectroscopy ( 1 H NMR), isothermal titration calorimetry and electrospray ionisation mass spectrometry. The 2PF@Q[8] complex was shown to be suitable for imaging gefitinib in prostate cancer (PC3) cells, which may help to elucidate relevant biological processes at the molecular level. We have developed a novel F-IDA to detect the anticancer drug GEF with high selectivity. The new indicator has excellent selectivity and a low detection limit for GEF. We have also demonstrated that the F-IDA can be used for the practical determination of drugs in living cells. Abstract :