Regeneration in bipinnaria larvae of the bat star Patiria miniata induces rapid and broad new gene expression. (November 2016)

• Record Type: Journal Article
• Authors: Oulhen, Nathalie; Heyland, Andreas; Carrier, Tyler J.; Zazueta-Novoa, Vanesa; Fresques, Tara; Laird, Jessica; Onorato, Thomas M.; Janies, Daniel; Wessel, Gary
• Is Part Of: Mechanisms of development. Volume 142(2016)
• Page Start: 10
• Page End: 21
• Publication Date: 2016-11
• Subjects: Vasa -- SRAP -- Dysferlin -- Vitellogenin -- Transcription -- Repair -- Patterning; Developmental biology -- Periodicals; Molecular biology -- Periodicals; Developmental Biology -- Periodicals; Molecular Biology -- Periodicals; Biologie du développement -- Périodiques; Biologie moléculaire -- Périodiques; Developmental biology; Molecular biology; Periodicals; Electronic journals; 571.8
• DOI: 10.1016/j.mod.2016.08.003
• Languages: English
• ISSNs: 0925-4773

Abstract

Abstract: Background: Some metazoa have the capacity to regenerate lost body parts. This phenomenon in adults has been classically described in echinoderms, especially in sea stars (Asteroidea). Sea star bipinnaria larvae can also rapidly and effectively regenerate a complete larva after surgical bisection. Understanding the capacity to reverse cell fates in the larva is important from both a developmental and biomedical perspective; yet, the mechanisms underlying regeneration in echinoderms are poorly understood. Results: Here, we describe the process of bipinnaria regeneration after bisection in the bat star Patiria miniata . We tested transcriptional, translational, and cell proliferation activity after bisection in anterior and posterior bipinnaria halves as well as expression of SRAP, reported as a sea star regeneration associated protease (Vickery et al., 2001b). Moreover, we found several genes whose transcripts increased in abundance following bisection, including: Vasa, dysferlin, vitellogenin 1 and vitellogenin 2. Conclusion: These results show a transformation following bisection, especially in the anterior halves, of cell fate reassignment in all three germ layers, with clear and predictable changes. These results define molecular events that accompany the cell fate changes coincident to the regenerative response in echinoderm larvae. Highlights: Bisected sea star larvae regenerate normal larval tissues and functions in a rapid and predictable fashion. Broad cellular replication accompanies regeneration in bisected larvae. Several genes were induced for over-expression in bisected larval fragments, including Vasa, dysferlin, and vitellogenin.