The relationship between capsular type and OmpA of Pasteurella multocida is associated with the outcome of disease. (December 2016)

• Record Type: Journal Article
• Authors: Verma, Subhash; Salwan, Richa; Katoch, Shailja; Verma, Lovit; Chahota, Rajesh; Dhar, Prasenjit; Sharma, Mandeep
• Is Part Of: Microbial pathogenesis. Volume 101(2016)
• Page Start: 68
• Page End: 75
• Publication Date: 2016-12
• Subjects: Pasteurella multocida -- OmpA -- Phylogenetic -- Transmembrane; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.11.003
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The genes encoding OmpA of Pasteurella multocida recovered from diseased and apparently healthy animals have been characterized. The nucleotide sequence revealed ORFs of 1047–1077 bp encoding proteins of 349–360 amino acids. Domain analysis of OmpA showed signal peptide, N-terminal ompA domain and C-terminal ligand binding domain. The transmembrane topology of OmpA showed short turns at the periplasmic end and longer irregular loops at the extracellular end. The phylogenetic analysis based on OmpA showed affiliation of isolates to 7 groups representing different alleles. The identical segments in OmpA also suggested assortative recombination within classes IV, V and VI of distinct lineages. Principal component analysis separated isolates into groups based on capsular type and PmompA alleles. The alleles belonging to class VI exclusively associated with capsular type A, whereas class I-IV were associated with capsular type B. PmompA alleles in class V were recorded in both serogroups. PmompA6.1, 6.4 were distributed among strains with capsular type A, and PmompA6.2 and 6.3 among capsular type B. Despite internal OmpA variabilty, restrictive and well defined distribution was seen amongst P. multocida . A definitive association of "OmpA-capsular type" was observed with clinical status of animals. A cohort of pasteurellae comprising of OmpA (I-IV)-capB was recovered from diseased animals and OmpA(VI)-capA from healthy subjects. This study concludes that P. multocida with serogroup A and B from healthy and diseased animals represent distinct clusters also differentiated based on their OmpA -types and OmpA -capsular type relationship possibly determine the virulence and disease outcome. Highlights: Transmembrane topology. 3D structure of OmpA. Capsule and OmpA profile. Existence of allele types.