Engineering RNA‐binding proteins for biology. (5th July 2013)

• Record Type: Journal Article
• Authors: Chen, Yu; Varani, Gabriele
• Is Part Of: FEBS journal. Volume 280:Number 16(2013)
• Page Start: 3734
• Page End: 3754
• Publication Date: 2013-07-05
• Subjects: computational design -- in vitro evolution -- phage display -- protein engineering -- protein–RNA interactions -- RNA recognition motif -- RNA‐binding domains -- RNA‐binding proteins -- yeast three‐hybrid system -- zinc finger; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.12375
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : RNA‐binding proteins play essential roles in the regulation of gene expression. Many have modular structures and combine relatively few common domains in various arrangements to recognize RNA sequences and/or structures. Recent progress in engineering the specificity of the PUF class RNA‐binding proteins has shown that RNA‐binding domains may be combined with various effector or functional domains to regulate the metabolism of targeted RNAs. Designer RNA‐binding proteins with tailored sequence specificity will provide valuable tools for biochemical research as well as potential therapeutic applications. In this review, we discuss the suitability of various RNA‐binding domains for engineering RNA‐binding specificity, based on the structural basis for their recognition. We also compare various protein engineering and design methods applied to RNA‐binding proteins, and discuss future applications of these proteins. Abstract : RNA‐binding proteins play essential roles in the regulation of gene expression. Many have modular structures and combine relatively few common domains to recognize RNA sequences and/or structures. Based on their structures, the suitability of different RNA‐binding domains for engineering RNA‐binding specificity is discussed here. Designer RNA‐binding proteins will provide valuable tools for biochemical research as well as potential therapeutic applications.