Detection and prevalence of boid inclusion body disease in collections of boas and pythons using immunological assays. (December 2016)

• Record Type: Journal Article
• Authors: Chang, L.; Fu, D.; Stenglein, M.D.; Hernandez, J.A.; DeRisi, J.L.; Jacobson, E.R.
• Is Part Of: Veterinary journal. Volume 218(2016)
• Page Start: 13
• Page End: 18
• Publication Date: 2016-12
• Subjects: Boid inclusion body disease -- Immunohistochemistry -- Snakes -- Reptarenavirus -- Nucleoprotein; Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2016.10.006
• Languages: English
• ISSNs: 1090-0233

Abstract

Highlights: Reptarenaviral nucleoprotein was identified in white blood cells of boa constrictors by immunohistochemistry. The overall prevalence of Inclusion Body Disease in the studied snakes was 19%. Boa constrictors were more commonly infected than other species in this study. Eighty-seven percent of IBD positive boa constrictors were clinically healthy. Abstract: Inclusion body disease (IBD) of boas and pythons is characterized by the intracytoplasmic accumulation of an antigenic 68 kDa viral protein IBDP, more recently known as the nucleoprotein (NP) of the reptarenaviruses. Blood samples of 131 captive boas and pythons (53 boa constrictors, Boa constrictor ; 35 rainbow boas, Epicrates cenchria ; 22 ball pythons, Python regius ; 5 carpet pythons, Morelia spilota ; 6 Burmese pythons, Python bivittatus ; 4 Jamaican boas, Epicrates subflavus ; 5 anacondas, Eunectes spp. ; and 1 green tree python, Morelia viridis ) were obtained from 28 collections in the USA. Diagnosis of IBD was initially made by the identification of eosinophilic intracytoplasmic inclusion bodies in hematoxylin and eosin (HE) stained blood films and isolated peripheral white blood cells (PWBC). The overall prevalence of IBD in study snakes was 25/131 or 19% (95% CI = 12.4%, 25.8%) with boa constrictors being more commonly infected (22/53 or 41.5%; 95% CI = 28.2%, 54.8%) than other species in this study. Of the 22 IBD positive boa constrictors, 87% were clinically healthy, 13% had various signs of chronic illness, and none showed signs of central nervous system disease. Using a validated monoclonal anti-NP antibody, NP was confirmed within the isolated PWBC by immunohistochemical staining and Western blots. The presence of reptarenaviruses within blood samples of 27 boa constrictors and three rainbow boas was also assessed by PCR. Among boa constrictors, very good agreements were shown between the observation of inclusion bodies (by HE stain) and the presence of NP (by immunohistochemistry, kappa = 0.92; and Western blots, kappa = 0.89), or the presence of reptarenaviruses (by PCR; kappa = 0.92).