Expression of globular form acetylcholinesterase is not altered in P2Y1R knock-out mouse brain. (25th November 2016)

• Record Type: Journal Article
• Authors: Cui, Jane D.; Xu, Miranda L.; Liu, Etta Y.L.; Dong, Tina T.X.; Lin, H.Q.; Tsim, Karl W.K.; Bi, Cathy W.C.
• Is Part Of: Chemico-biological interactions. Volume 259:Part B(2016)
• Page Start: 291
• Page End: 294
• Publication Date: 2016-11-25
• Subjects: AChE -- ACh -- P2Y1 receptor -- Neuron growth; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2016.06.028
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Adenosine 5′-triphosphate (ATP), a neurotransmitter and a neuromodulator, has been shown to be co-stored and co-released with acetylcholine (ACh) at the pre-synaptic vesicles in vertebrate neuromuscular junction (nmj). Several lines of studies demonstrated that binding of ATP to its corresponding P2Y1 receptors (P2Y1 R) in muscle and neuron regulated the post-synaptic gene expressions. Indeed, the expression of acetylcholinesterase (AChE) in muscle was markedly decreased in P2Y1R −/− (P2Y1 R knock-out) mice. In order to search for possible role of P2Y1 R in cholinergic function of the brain, the expression of globular form AChE was determined in the brain of P2Y1R −/− mice. In contrast to that in muscle, the amounts of AChE activity, AChE catalytic subunit, structure subunit PRiMA and the amount of ACh, in the brain were not, significantly, altered, suggesting the role of P2Y1 R in neuron could have different function as that in muscle. However, the expressions of a series of neuronal development markers, i.e. neurofilaments, were reduced in P2Y1R −/− mouse brain, indicating P2Y1 R may be involved in neuronal development process. Highlights: Dysfunction of P2Y1 R did not alter the expressions of G4 AChE in mouse brain. Dysfunction of P2Y1 R did not affect the amount of ACh in mouse brain. Reduced expressions of neurofilaments were found in P2Y1R −/− mouse brain. ATP/ P2Y1R signaling may play roles in neuronal differentiation.