Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. (December 2016)

• Record Type: Journal Article
• Authors: Tebianian, Majid; Mosavari, Nader; Taghizadeh, Morteza; Ebrahimi, Seyyed Mahmoud
• Is Part Of: International journal of mycobacteriology. Volume 5 (2016:Dec.)Supplement 1
• Page Start: S254
• Publication Date: 2016-12
• Subjects: Mycobacterium tuberculosis -- ESAT-6 -- CFP-10 -- Specific antibodies -- ELISA; Mycobacteria -- Periodicals; Mycobacterial diseases -- Periodicals; Mycobacteriaceae; Mycobacteria; Electronic journals; Periodicals; 579.374
• DOI: 10.1016/j.ijmyco.2016.11.011
• Languages: English
• ISSNs: 2212-5531

Abstract

Abstract: Introduction: Since an accurate test for detection of Mycobacterium tuberculosis early infection is urgently needed, this study was designed for development of an efficient screening test in diagnosis of tuberculosis infection. Materials and methods: In the present study, two recombinant proteins CFP-10, ESAT-6 were tested as antigens for the diagnosis of recent tuberculosis. The proteins were produced in Escherichia coli, purified and tested in indirect ELISAs with sera from 63 subjects with positive clinical results. Also, 56 sera from healthy persons were tested as controls. The results were compared with molecular and culture. Results: The levels of antibodies against M. tuberculosis antigens in patients with tuberculosis were significantly higher than those in healthy subjects. Among 63 patients, 58 were positive for ESAT-6, 54 for CFP-10. Conclusion: Altogether, the role of M. tuberculosis recombinant proteins, as a suitable candidate for early diagnosis of tuberculosis infection was supported in this study. However, these strongly offer the potential of mixture or fusion of these recombinant proteins for better sensitivity and specificity.