Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective. Issue 3 (1st March 2016)

• Record Type: Journal Article
• Authors: Owen, Robin L.; Sherrell, Darren A.
• Is Part Of: Acta crystallographica. Volume 72:Issue 3(2016)
• Page Start: 388
• Page End: 394
• Publication Date: 2016-03-01
• Subjects: phasing -- radiation damage -- macromolecular crystallography; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798315021555
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : Both site‐specific radiation damage and heavy‐atom derivatization result in small changes to the intensity of reflections. The size of the change owing to each is calculated and compared for individual reflections. Abstract : During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site‐specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site‐specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X‐ray‐induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.