Atomic resolution experimental phase information reveals extensive disorder and bound 2‐methyl‐2, 4‐pentanediol in Ca2+ 2+‐calmodulin. Issue 1 (1st January 2016)

• Record Type: Journal Article
• Authors: Lin, Jiusheng; van den Bedem, Henry; Brunger, Axel T.; Wilson, Mark A.
• Is Part Of: Acta crystallographica. Volume 72:Issue 1(2016)
• Page Start: 83
• Page End: 92
• Publication Date: 2016-01-01
• Subjects: experimental phases experimental phases -- disorder disorder -- qFit qFit -- density modification density modification; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798315021609
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : Experimental phase information at 1.0 Å resolution was used to validate a more complete multiple‐conformation model of disorder in Ca 2+ 2+ ‐calmodulin and identified bound 2‐methyl‐2, 4‐pentanediol (MPD) in multiple regions of the protein. This bound MPD is likely to contribute to some of the previously noted peculiar features of the Ca 2+ 2+ ‐calmodulin crystal structure. Abstract : Calmodulin (CaM) is the primary calcium signaling protein in eukaryotes and has been extensively studied using various biophysical techniques. Prior crystal structures have noted the presence of ambiguous electron density in both hydrophobic binding pockets of Ca 2+ 2+ ‐CaM, but no assignment of these features has been made. In addition, Ca 2+ 2+ ‐CaM samples many conformational substates in the crystal and accurately modeling the full range of this functionally important disorder is challenging. In order to characterize these features in a minimally biased manner, a 1.0 Å resolution single‐wavelength anomalous diffraction data set was measured for selenomethionine‐substituted Ca 2+ 2+ ‐CaM. Density‐modified electron‐density maps enabled the accurate assignment of Ca 2+ 2+ ‐CaM main‐chain and side‐chain disorder. These experimental maps also substantiate complex disorder models that were automatically built using low‐contour features of model‐phased electron density. Furthermore, experimental electron‐density maps reveal that 2‐methyl‐2, 4‐pentanediol (MPD) is present in the C‐terminal domain, mediates a lattice contact between N‐terminal domains and may occupy the N‐terminal binding pocket. The majority of the crystal structures of target‐free Ca 2+ 2+ ‐CaM have been derived from crystals grown using MPD as a precipitant, and thus MPD is likely to be bound in functionally critical regions of Ca 2+ 2+ ‐CaM in most of these structures. The adventitious binding of MPD helps to explain differences between the Ca 2+ 2+ ‐CaM crystal and solution structures and is likely to favor more open conformations of the EF‐hands in the crystal.