Dietary protein enhances non-specific immunity, anti-oxidative capability and resistance to Aeromonas hydrophila in Labeo rohita fingerlings pre-exposed to short feed deprivation stress. (December 2016)

• Record Type: Journal Article
• Authors: Yengkokpam, Sona; Debnath, Dipesh; Sahu, N.P.; Pal, A.K.; Jain, K.K.; Baruah, Kartik
• Is Part Of: Fish & shellfish immunology. Volume 59(2016:Dec.)
• Page Start: 439
• Page End: 446
• Publication Date: 2016-12
• Subjects: Labeo rohita -- Feed deprivation -- Growth -- Immune response -- Disease resistance -- Anti-oxidative enzymes; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.10.052
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Present experiment was conducted to study the effect of dietary protein levels on growth, immunity and anti-oxidative status of Labeo rohita fingerlings during feed deprivation followed by refeeding. Fish (5.44 ± 0.10 g) were deprived of feed for 3 weeks and then re-fed to satiation for 5 weeks with one of the diets containing 25 (25P), 30 (30P), 35 (35P) or 40 (40P) percent crude protein (CP) level. In addition to these groups, a control group (C) was also maintained by feeding to satiation level twice daily with a diet containing 30% CP throughout the experimental period. At the end of 8-weeks' trial, fish were challenged with Aeromonas hydrophila and survival was recorded for the next 7 days. Complete recovery of growth in terms of weight gain percentage was achieved in the fish fed 35 and 40% protein during refeeding. The body indices (condition factor and hepatosomatic index), haematological parameters and serum protein contents at the end of the experimental trial were not significantly different (P > 0.05) among different groups suggesting that the overall health of the fish was not compromised. However, respiratory burst activity and serum lysozyme activity were indicative of a better immune function in the higher protein fed groups (35P and 40P) than the lower protein groups (25P and 30P). Following challenge with Aeromonas hydrophila, survival rate, blood monocyte%, respiratory burst activity, serum lysozyme activity, serum protein and globulin were significantly higher (P < 0.05) in the 35P and 40P groups compared to the other groups. Further, fish fed lower dietary protein were not able to restore the activities of anti-oxidative enzymes (superoxide dismutase and catalase) in the liver. Conclusively, an improved disease resistance capability and immune status was observed in the fish fed a higher dietary protein (35–40%), even out-performing the daily-fed fish. Highlights: Health of fish was not compromised by a 3-week feed deprivation and 5-week refeeding protocol. Refeeding with higher dietary protein led to complete compensatory growth. Higher protein diet improved the disease resistant capability and immune status of the fish. Fish fed with sub-optimal protein were not able to restore the anti-oxidative enzyme activities.