Characterization of microRNAs by deep sequencing in red claw crayfish Cherax quadricarinatus haematopoietic tissue cells after white spot syndrome virus infection. (December 2016)

• Record Type: Journal Article
• Authors: Zhao, Meng-ru; Meng, Chuang; Xie, Xiao-lu; Li, Cheng-hua; Liu, Hai-peng
• Is Part Of: Fish & shellfish immunology. Volume 59(2016:Dec.)
• Page Start: 469
• Page End: 483
• Publication Date: 2016-12
• Subjects: White spot syndrome virus -- Crayfish haematopoietic tissue -- MicroRNA -- Innate immunity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.11.012
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: White spot syndrome virus (WSSV) is one of the most prevalent and widespread viruses in both shrimp and crayfish aquaculture. MicroRNAs (miRNAs) are crucial post-transcriptional regulators and play critical roles in cell differentiation and proliferation, apoptosis, signal transduction and immunity. In this study, miRNA expression profiles were identified via deep sequencing in red claw crayfish Cherax quadricarinatus haematopoietic tissue (Hpt) cell cultures infected with WSSV at both early (i.e., 1 hpi) and late (i.e., 12 hpi) infection stages. The results showed that 2 known miRNAs, namely, miR-7 and miR-184 play key roles in immunity. Meanwhile, 106 novel miRNA candidates were predicted by software in these combined miRNA transcriptomes. Compared with two control groups, 36 miRNAs showed significantly different expression levels after WSSV challenge. Furthermore, 10 differentially expressed miRNAs in WSSV-exposed Hpt cells were randomly selected for expression analysis by quantitative real-time RT-PCR. Consistent with the expression profiles identified by deep sequencing, RT-PCR showed a significant increase or decrease in miRNA expression in Hpt cells after WSSV infection. Prediction of targets of miRNAs such as miR-7, cqu-miR-52, cqu-miR-126 and cqu-miR-141 revealed that their target genes have diverse biological roles, including not only immunity but also transcriptional regulation, energy metabolism, cell communication, cell differentiation, cell death, autophagy, endocytosis and apoptosis. These results provide insight into the molecular mechanism of WSSV infection and highlight the function of miRNAs in the regulation of the immune response against WSSV infection in crustaceans. Highlights: Both known and novel miRNAs were identified from red claw crayfish Hpt cell cultures infected with WSSV. Thirty-six host miRNAs showed significantly different expression post WSSV challenge. Differentially expressed miRNAs target diverse key biological processes including autophagy, endocytosis and apoptosis. Shed new light on the miRNAs in the regulation of the immune response against WSSV infection in crustaceans.