Vegetable oil induced inflammatory response by altering TLR-NF-κB signalling, macrophages infiltration and polarization in adipose tissue of large yellow croaker (Larimichthys crocea). (December 2016)

• Record Type: Journal Article
• Authors: Tan, Peng; Dong, Xiaojing; Mai, Kangsen; Xu, Wei; Ai, Qinghui
• Is Part Of: Fish & shellfish immunology. Volume 59(2016:Dec.)
• Page Start: 398
• Page End: 405
• Publication Date: 2016-12
• Subjects: Fish oil -- Vegetable oil -- Immunity -- Adipose tissue -- Large yellow croaker; TLR toll-like receptor -- NF-κB nuclear factor kappa beta -- RB respiratory burst -- LZM lysozyme -- ACP alternative complement pathway -- CMR cumulative mortality rate -- DHA ducosahexenoic acid -- EPA eicosapentaenoic acid -- AT adipose tissue -- IKKα/β inhibitor of NF-κB kinase α/β -- IκBα inhibitor of NF-κB -- CD68 cluster of differentiation 68 -- CSF1R colony-stimulating factor 1 receptor -- MRC1 macrophage mannose receptor 1 -- CD209 cluster of differentiation 209 -- IL1β interleukin 1β -- TNFα tumour necrosis factor α -- Arg I arginase I -- IL10 interleukin 10 -- GAPDH glyceraldehyde-3-phosphate dehydrogenase; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.11.009
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: High level of vegetable oil (VO) in diets could induce strong inflammatory response, and thus decrease nonspecific immunity and disease resistance in most marine fish species. The present study was conducted to investigate whether dietary VO could exert these anti-immunological effects by altering TLR-NF-κB signalling, macrophages infiltration and polarization in adipose tissue of large yellow croaker ( Larimichthys crocea ). Three iso-nitrogenous and iso-lipid diets with 0% (FO, fish oil, the control), 50% (FV, fish oil and vegetable oil mixed) and 100% (VO, vegetable oil) vegetable oil were fed to fish with three replicates for ten weeks. The results showed that activities of respiratory burst (RB) and alternative complement pathway (ACP), as well as disease resistance after immune challenge were significantly decreased in large yellow croaker fed VO diets compared to FO diets. Inflammatory response of experimental fish was markedly elevated by VO reflected by increase of pro-inflammatory cytokines (IL1β and TNFα) and decrease of anti-inflammatory cytokine (arginase I and IL10) genes expression. TLR-related genes expression, nucleus p65 protein, IKKα/β and IκBα phosphorylation were all significantly increased in the AT of large yellow croaker fed VO diets. Moreover, the expression of macrophage infiltration marker proteins (cluster of differentiation 68 [CD68] and colony-stimulating factor 1 receptor [CSF1R]) was significantly increased while the expression of anti-inflammatory M2 macrophage polarization marker proteins (macrophage mannose receptor 1 [MRC1] and cluster of differentiation 209 [CD209]) was significantly decreased in the AT of large yellow croaker fed VO diets. In conclusion, VO could induce inflammatory responses by activating TLR-NF-κB signalling, increasing macrophage infiltration into adipose tissue and polarization of macrophage in large yellow croaker. Highlights: Dietary vegetable oils induce inflammatory responses in adipose tissue of large yellow croaker. Inflammatory responses induced by vegetable oils in adipose tissue are related to activating of TLR-NF-κB signalling. Dietary vegetable oils increase macrophage infiltration into adipose tissue and polarization of macrophage.