MicroRNA-guided diagnostics in clinical samples. Issue 5 (October 2016)

• Record Type: Journal Article
• Authors: Gustafson, Dakota; Tyryshkin, Kathrin; Renwick, Neil
• Is Part Of: Best practice & research. Volume 30:Issue 5(2016)
• Page Start: 563
• Page End: 575
• Publication Date: 2016-10
• Subjects: miRNA-guided diagnostics -- miRNA -- microRNA -- biomarkers -- detection methods -- clinical decision-making; Endocrine glands -- Diseases -- Periodicals; Metabolism -- Disorders -- Periodicals; Endocrinology -- Periodicals; Metabolism -- Periodicals; 616.4
• DOI: 10.1016/j.beem.2016.07.002
• Languages: English
• ISSNs: 1521-690X

Abstract

Abstract : miRNA-guided diagnostics is a powerful molecular approach for evaluating clinical samples through miRNA detection and/or visualization. To date, this approach has been successfully used to diagnose, manage, and/or monitor a wide range of neoplastic and non-neoplastic diseases. Despite the promise of miRNA-guided diagnostics, particularly in the field of minimally invasive biomarkers, several knowledge and practical issues confound or hinder translation into routine clinical practice including: miRNA sequence database errors, suboptimal RNA extraction methods, detection assay variability, a vast array of online resources for bioinformatic analyses, and non-standardized statistical analyses for miRNA clinical testing. In this review, we raise awareness of these issues and recommend research directions to help specialists in endocrinology and metabolism integrate miRNA testing into clinical decision-making.