A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice. (20th December 2016)

• Record Type: Journal Article
• Authors: Yang, Rui; Li, Yafei; Su, Yan; Shen, Yi; Tang, Ding; Luo, Qiong; Cheng, Zhukuan
• Is Part Of: Journal of genetics and genomics. Volume 43:Number 12(2016)
• Page Start: 694
• Page End: 701
• Publication Date: 2016-12-20
• Subjects: Rice -- Ring chromosome -- Meiosis -- Centromere; Genetics -- Periodicals; Genomics -- Periodicals; 576.505
• DOI: 10.1016/j.jgg.2016.09.006
• Languages: English
• ISSNs: 1673-8527

Abstract

Abstract: An awned rice ( Oryza sativa ) plant carrying a tiny extra chromosome was discovered among the progeny of a telotrisomic line 2n+4L. Fluorescence in situ hybridization (FISH) using chromosome specific BAC clones revealed that this extra chromosome was a ring chromosome derived from part of the long arm of chromosome 4. So the aneuploidy plant was accordingly named as 2n+4L ring. We did not detect any CentO FISH signals on the ring chromosome, and found only the centromeric probe Centromeric Retrotransposon of Rice (CRR) was co-localized with the centromere-specific histone CENH3 as revealed by sequential FISH after immunodetection. The extra ring chromosome exhibited a unique segregation pattern during meiosis, including no pairing between the ring chromosome and normal chromosome 4 during prophase I and pre-separation of sister chromatids at anaphase I.