Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas). Issue 60 (January 2017)

• Record Type: Journal Article
• Authors: Cong, Bailin; Chai, Yingmei; Wang, Bo; Liu, Shenghao; Shen, Jihong; Wang, Nengfei; Zhang, Quanqi; Huang, Xiaohang
• Is Part Of: Fish & shellfish immunology. Issue 60(2017)
• Page Start: 426
• Page End: 435
• Publication Date: 2017-01
• Subjects: Voltage-gated potassium ion channels -- Sea perch Lateolabrax japonicas -- Macrophages -- LPS -- Immune function; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.09.040
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Potassium ion channels are one of the most diversely and widely distributed channels, which are involved in all kinds of physiological functions in both excitable and non-excitable cells. The expression of voltage-gated potassium ion (Kv) channels is highly variable according to the state of macrophages activation. Macrophages have an important function in innate immunity against intruding pathogens. They produce a variety of inflammatory and immunoactive molecules that modulate imflammatory responses. Here we show that blockade of K + channels by non-selective Kv channel inhibitor tetraethylammonium chloride (TEA), and 4-aminopyridine (4-AP) inhibited proinflammatory cytokines expression, cell proliferation, and reactive oxygen species (ROS) production in LPS-stimulated macrophages of Sea perch ( Lateolabrax japonicas ). Then we isolated four Kv channels genes (spKv1.1, spKv1.2, spKv1.5 and spKv3.1) in LPS-activated fish macrophages. These channels genes were up-regulated after LPS stimulation except spKv3.1, which remained unchanged during the test. The results of this study indicate that Kv channels could be required for modulating the immune function of fish macrophages. Highlights: Isolate and characterize four voltage-gated potassium channels genes from macrophages of Sea perch. K + channel blockers downregulated TNF-α and IL1-β in LPS-induced macrophages. K + channel blocker suppressed the ROS production and proliferation of LPS-activated macrophages. Most of Kv we found may play an important role in modulating the immune function of Sea perch macrophages.