Characterization of a specific monoclonal antibody against immunoglobulin light kappa/L1 chain in olive flounder (Paralichthys olivaceus). Issue 60 (January 2017)

• Record Type: Journal Article
• Authors: Kim, Young Kyu; Lee, Jung Seok; Jung, Jae Wook; Hikima, Jun-ichi; Ohtani, Maki; Jang, Ho Bin; Nho, Seong Won; Cha, In Seok; Park, Seong Bin; Lee, Jeong Ho; Aoki, Takashi; Jung, Tae Sung
• Is Part Of: Fish & shellfish immunology. Issue 60(2017)
• Page Start: 88
• Page End: 96
• Publication Date: 2017-01
• Subjects: Immunoglobulin (Ig) -- Igκ-a -- Monoclonal antibodies (mAbs) -- Olive flounder; Ig Immunoglobulin -- IgL Immunoglobulin light chain -- mAb Monoclonal antibody -- MALDI-TOF Matrix assisted laser desorption/ionization time-of-flight -- FACS Fluorescence-activated cell sorting -- RT-PCR Reverse transcription-polymerase chain reaction -- CDR Complementarity-determining region -- FR Framework region -- PBL Peripheral blood lymphocyte; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.11.023
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Immunoglobulins (Ig) are heterodimeric proteins that play critical roles in the adaptive immune system of vertebrates. Because of their plasticity, teleostean Igs are more diverse, and thus do not conform to mammalian classifications. Because of this, mammalian-based Ig cell markers cannot be used successfully to study immune responses in fish. There is therefore a need to produce Ig-specific cell markers for fish. Here, we attempted to identify the specific isotype detected by an Ig light chain-specific monoclonal antibody (anti-olive flounder IgL-mAb: M7C3-4) that we had previously produced [11]. Three newly identified sequences of the Ig light chain from olive flounder were classified according to their isotypes. Subsequent analyses revealed that M7C3-4 was able to specifically detect lymphocytes expressing one of the κ chains (Igκ-a) in olive flounder. Interestingly, Igκ-a + B cells were more abundant in spleen and trunk-kidney than in peripheral blood, indicating a distribution different from that of IgM + B cells. Our work reveals interesting aspects of B cell distribution and differentiation, and may aid in the production of suitable and effective cell markers for olive flounder. Highlights: Olive flounder has three isotypes of IgL, two of κ, and one of σ. M7C3-4 produced against serum immunoglobulins of olive flounder specifically recognizes Igκ-a isotype. Igκ-a+ B cells are more abundant in organs than in peripheral blood.