A novel C-type lectin in the black tiger shrimp Penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination. Issue 60 (January 2017)

• Record Type: Journal Article
• Authors: Wongpanya, Ratree; Sengprasert, Panjana; Amparyup, Piti; Tassanakajon, Anchalee
• Is Part Of: Fish & shellfish immunology. Issue 60(2017)
• Page Start: 103
• Page End: 113
• Publication Date: 2017-01
• Subjects: C-type lectin -- Penaeus monodon -- Innate immunity -- Pattern recognition receptor; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.11.042
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: C-type lectins are pattern recognition proteins that play important roles in innate immunity in invertebrates by mediating the recognition of pathogens. In this study, a novel C-type lectin gene, Pm CLec, was cloned and characterized from the black tiger shrimp Penaeus monodon . The open reading frame of Pm CLec is 657 bp in length. It encodes a predicted protein of 218 amino acids with a calculated molecular mass and an isoelectric point of 24086 Da and 4.67, respectively. Sequence analysis of Pm CLec showed similarity to members of the C-type lectin gene superfamily. The deduced protein contains a single carbohydrate recognition domain (CRD) and four conserved cysteine residues (Cys 58, Cys 126, Cys 141, Cys 149 ) that are involved in the formation of disulfide bridges. Pm CLec transcripts are expressed in various tiger shrimp tissues, with the highest expression in the lymphoid organ. RNAi-mediated silencing of Pm CLec resulted in higher cumulative mortality of knockdown shrimp after Vibrio harveyi infection compared to the control groups. Recombinant Pm CLec was successfully expressed in the E. coli system. In the presence of Ca 2+, purified r Pm CLec protein binds and agglutinates Gram-positive bacteria ( Staphylococcus aureus, S. hemolyticus ), but only slightly binds and agglutinates E. coli and could not bind to the Gram-negative bacteria Bacillus megaterium and Vibrio harveyi . These results suggest that Pm CLec functions as a pattern recognition receptor that is implicated in shrimp innate immunity. Highlights: C-type lectin gene, Pm CLec, was cloned and characterized from the black tiger shrimp Penaeus monodon. Cumulative mortality of Pm CLec knockdown shrimp was high after Vibrio harveyi infection. r Pm CLec binds and agglutinates Staphylococcus aureus, S. hemolyticus, but only slightly binds and agglutinates E. coli .