Basagran® induces developmental malformations and changes the bacterial community of zebrafish embryos. (February 2017)

• Record Type: Journal Article
• Authors: Oliveira, Jacinta M.M.; Galhano, Victor; Henriques, Isabel; Soares, Amadeu M.V.M.; Loureiro, Susana
• Is Part Of: Environmental pollution. Volume 221(2017)
• Page Start: 52
• Page End: 63
• Publication Date: 2017-02
• Subjects: Basagran® -- Zebrafish -- Embryotoxicity -- Bacterial communities -- DGGE; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2016.10.028
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: This study aimed to assess the effects of Basagran ® on zebrafish ( Danio rerio ) embryos. The embryos were exposed to Basagran ® at concentrations ranging from 120.0 to 480.6 mg/L, and the effects on embryo development (up to 96 h) and bacterial communities of 96 h-larvae were assessed. The embryo development response was time-dependent and concentration-dependent (106.35 < EC50 < 421.58 mg/L). The sensitivity of embryo-related endpoints decreased as follows: blood clotting in the head and/or around the yolk sac > delay or anomaly in yolk sac absorption > change in swimming equilibrium > development of pericardial and/or yolk sac oedema > scoliosis. A PCR-DGGE analysis was used to evaluate changes in the structure, richness, evenness and diversity of bacterial communities after herbicide exposure. A herbicide-induced structural adjustment of bacterial community was observed. In this study, it was successfully demonstrated that Basagran ® affected zebrafish embryos and associated bacterial communities, showing time-dependent and concentration-dependent embryos' developmental response and structural changes in bacterial community. Thus, this work provides for the first time a complementary approach, which is useful to derive robust toxicity thresholds considering the embryo-microbiota system as a whole. The aquatic hazard assessment will be strengthened by combining current ecotoxicological tests with molecular microbiology tools. Graphical abstract: Highlights: This study highlights Basagran ® as an important stressor to zebrafish embryos. Basagran ® induced developmental effects on zebrafish embryos. The structure of bacterial community associated with zebrafish embryos was affected. Abstract : Exposure of zebrafish embryos to Basagran ® affects their development and structurally changes their associated bacterial communities.