Molecular and functional characterization of pigeon (Columba livia) tumor necrosis factor receptor-associated factor 3. (April 2017)

• Record Type: Journal Article
• Authors: Zhou, Yingying; Kang, Xilong; Xiong, Dan; Zhu, Shanshan; Zheng, Huijuan; Xu, Ying; Guo, Yaxin; Pan, Zhiming; Jiao, Xinan
• Is Part Of: Developmental and comparative immunology. Volume 69(2017)
• Page Start: 51
• Page End: 59
• Publication Date: 2017-04
• Subjects: Pigeon -- Tumor necrosis factor receptor-associated factor 3 -- Cloning -- Function -- Identification; PRR pattern recognition receptor -- TLR Toll-like receptor -- RLR RIG-I-like receptor -- NLR NOD-like receptor -- DC dendritic cell -- TNFR tumor necrosis factor receptor -- TRAF TNFR-associated factor -- MEF mouse embryonic fibroblast -- IFN interferon -- NIK NF-κB-inducing kinase -- PiTRAF3 pigeon TRAF3 -- PBMC peripheral blood mononuclear cell -- LPS lipopolysaccharide -- ORF open reading frame -- SMART Simple Modular Architecture Research Tool -- RE response element -- ODN oligodeoxynucleotides -- RT-PCR reverse transcription PCR -- qPCR quantitative real-time PCR -- IKK inhibitor of κB kinase -- LsTRAF3 Lutjanus sanguineus TRAF3 -- FHM fathead minnow; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.12.005
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Tumor necrosis factor receptor-associated factor 3 (TRAF3) plays a key antiviral role by promoting type I interferon production. We cloned the pigeon TRAF3 gene ( PiTRAF3 ) according to its predicted mRNA sequence to investigate its function. The 1704-bp full-length open reading frame encodes a 567-amino acid protein. One Ring finger, two TRAF-type Zinc fingers, one Coiled coil, and one MATH domain were inferred. RT-PCR showed that PiTRAF3 was expressed in all tissues, with relatively weak expression in the heart and liver. In HEK293T cells, over-expression of wild-type, △ Ring, △ Zinc finger, and △ Coiled coil PiTRAF3, but not a △ MATH form, significantly increased IFN-β promoter activity. Zinc finger and Coiled coil domains were essential for NF-κB activation. In chicken HD11 cells, PiTRAF3 increased IFN-β promoter activity and four domains were all contributing. R848 stimulation of pigeon peripheral blood mononuclear cells and splenocytes significantly increased expression of PiTRAF3 and the inflammatory cytokine genes CCL5, IL-8, and IL-10 . These data demonstrate TRAF3's innate immune function and improve understanding of its involvement in poultry antiviral defense. Highlights: Pigeon TRAF3 was expressed in all tested tissues. The MATH domain is necessary for IFN-β promoter activation. The zinc finger and coiled coil are essential for NF-κB activation. R848 induces TRAF3 and cytokine up-regulation in pigeon PBMCs and splenocytes.