Cross‐protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv. (November 2016)

• Record Type: Journal Article
• Authors: Lee, Tae Hee; Cha, Sun‐Shin; Lee, Chang‐Seop; Rhee, Joon Haeng; Woo, Hye Ryun; Chung, Kyung Min
• Is Part Of: Microbiology and immunology. Volume 60:Number 11(2016:Nov.)
• Page Start: 793
• Page End: 800
• Publication Date: 2016-11
• Subjects: cross‐protection -- MARTX toxin -- monoclonal antibody -- Vibrio species; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12449
• Languages: English
• ISSNs: 0385-5600

Abstract

ABSTRACT: Gram‐negative Vibrio species secrete multifunctional autoprocessing repeats‐in‐toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross‐reactivity and cross‐protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro ; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.