Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect. (November 2016)

• Record Type: Journal Article
• Authors: Sato, Ko; Watanabe, Oshi; Ohmiya, Suguru; Chiba, Fumiko; Hayashi, Masahiro; Suzuki, Tamio; Kawakami, Kazuyoshi; Nishimura, Hidekazu
• Is Part Of: Microbiology and immunology. Volume 60:Number 11(2016:Nov.)
• Page Start: 801
• Page End: 805
• Publication Date: 2016-11
• Subjects: cytopathic effect -- human parainfluenza virus -- MNT‐1 cells -- virus isolation; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12446
• Languages: English
• ISSNs: 0385-5600

Abstract

ABSTRACT: Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens.