Polarized monolayer cultures of human intestinal epithelial cell lines exposed to intractable proteins - In vitro hazard identification studies. (December 2016)

• Record Type: Journal Article
• Authors: Hurley, Bryan P.; Eaton, Alex D.; Zimmermann, Cindi; Delaney, Bryan
• Is Part Of: Food and chemical toxicology. Volume 98:Part B(2016)
• Page Start: 262
• Page End: 268
• Publication Date: 2016-12
• Subjects: Intractable proteins -- Intestinal epithelial cells -- Cytotoxicity; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2016.11.006
• Languages: English
• ISSNs: 0278-6915

Abstract

Highlights: Intractable proteins did not produce adverse effects toward human intestinal epithelial cell (IEC) monolayers. Intractable proteins do not appear to be inherently hazardous as a class of proteins. In vitro methods of protein hazard identification require much less protein than studies with laboratory animals. Inexpensive and readily available cells, reagents, and instruments can be used for protein hazard identification.