Detection of AmpC β-lactamase and adherence factors in uropathogenic Escherichia coli isolated from aged patients. (November 2016)

• Record Type: Journal Article
• Authors: Singh, Santosh Kumar; Seema, Kumari; Gupta, Minakshi
• Is Part Of: Microbial pathogenesis. Volume 100(2016)
• Page Start: 293
• Page End: 298
• Publication Date: 2016-11
• Subjects: Uropathogenic Escherichia coli/UPEC -- Multiple drug resistance -- Virulence factors -- Beta-lactamases -- Biofilm; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.10.010
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Escherichia coli mediated urinary tract infection has been reported to be most prevalent among patients of different class, gender and ages. Currently, multidrug resistant E. coli harboring several virulence factors are most perilous threats for patients especially for elders. The aim of this study was to determine the antibiotic resistance pattern, co-resistance and phenotypic virulence factors present in uropathogenic E. coli isolated from aged patients. Thirty-nine E. coli isolates were collected during May–June 2014 from patients between 50 to 80 years of age. Experiments have been carried out to determine the antibiotic resistance, co-resistances and phenotypic adherent factors present in each isolate. Clonal relatedness was also determined in the Amp C positive uropathogenic E. coli (UPEC). 97.43% isolates were found to be multidrug resistant and 41.02% of them were Amp C producer. Amp C producer group showed higher multiple antibiotic resistance index than Amp C non-producer (p value < 0.01) group. Interestingly, adherence factor Type 1 fimbriae were found among 84.61% of total isolates which were more prevalent in elderly female patients than males. Biofilm production studies revealed that 84.61% of total isolates are more common in elderly males. This study adds value for the proper empiric selection of antibiotic therapy as well as calls for continuous monitoring of the incidence of drug resistance virulent uropathogenic E. coli mediated urinary tract infection in elderly patients. Highlights: Detection of Amp C and adherence factors in uropathogenic Escherichia coli (UPEC) isolated from the aged patients. Amp C producer UPEC strains displayed high MAR index than non-producers. Adherence factors Type-1 fimbriae was more prevalent in UPEC from female patients. Biofilm formation was more prevalent in UPEC from male patients.