Amino acid-based ionic liquid surface modification of magnetic nanoparticles for the magnetic solid-phase extraction of heme proteins. Issue 107 (4th November 2016)

• Record Type: Journal Article
• Authors: Wang, Binghai; Wang, Xiong; Wang, Juanqiang; Xue, Xue; Xi, Xingjun; Chu, Qiao; Dong, Genlai; Wei, Yun
• Is Part Of: RSC advances. Volume 6:Issue 107(2016)
• Page Start: 105550
• Page End: 105557
• Publication Date: 2016-11-04
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra23616b
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Amino acid-based ionic liquid functionalized magnetic nanoparticles were fabricated as an MSPE adsorbent for hemoglobin with a binding capacity of 1.58 g g −1 . Abstract : In this study, magnetic nanoparticles with an amino acid-based ionic liquid directly modified onto their surface were fabricated as a new magnetic adsorbent (Fe3 O4 @DIH–EMIMLpro) for the magnetic solid phase extraction of protein. The synthesized Fe3 O4 @DIH–EMIMLpro nanoparticles were characterized using Fourier transform infrared spectrometry, transmission electron microscopy, vibrating sample magnetometry, X-ray diffraction and thermogravimetric analysis. Hemoglobin was chosen as a model protein to investigate the extraction performance of the prepared materials. After extraction, the concentration of protein in the supernatant was determined by UV-vis spectrophotometry. The adsorbed hemoglobin could be eluted from the Fe3 O4 @DIH–EMIMLpro nanoparticles by sodium dodecyl sulfate solution. The Fe3 O4 @DIH–EMIMLpro nanoparticles could be used for rapid, efficient, and selective adsorption of hemoglobin with a binding capacity as high as 1.58 mg mg −1 . Moreover, the extraction process of protein from human whole blood by Fe3 O4 @DIH–EMIMLpro has been performed to evaluate the practical application of the proposed method.